【文獻標題】Course-, dose-, and stage-dependent toxic effects of prenatal dexamethasone exposure on fetal articular cartilage development
【作者】Ze Chen,Zhe Zhao,Yunzepeng Li,et.al
【作者單位】武漢大學基礎醫學院(Basic Medical School of Wuhan University)
【文獻中引用產品】
試劑-藏紅O
【關鍵詞】Prenatal exposure,Dexamethasone,Developmental toxicity,Articular cartilage development,Transforming growth factor beta
【DOI】doi.org/10.1016/j.toxlet.2018.01.008
【影響因子(IF)】5.70
【出版期刊】《Toxicology Letters》
【產品原文引用】
Dexamethasone (CAS. NO. 50-02-2, 1 ml: 5 mg, AR) was obtained from Kingyork Group Co., Ltd. (Tianjin, China). afranin-O (CAS. NO.477-73-6) was obtained from Hengyuan Biotech Co., Ltd. (Shanghai,China). Monoclonal mouse collagen type II alpha 1 (Col2a1) antibody (sc-52658, 100 μg/ml) was purchased from Santa Cruz Biotechnology,Inc. (Santa Cruz, CA, USA). Trizol reagent kits were obtained from Omega Bio-Tek (Doraville, USA). The SYBR Green dye was purchased from ABI (Foster City, CA, USA). Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). The oligonucleotide primers for mouse RT-qPCR genes (PAGE purification) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Other chemicals and agents were of analytical grade.
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